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	Provedor de dados Genet. Mol. Biol. (2) Acta Agron. (Palmira) (1) JBAG (1) Electron. J. Biotechnol. (1) Autor Postiglioni,Alicia (5) Rincón,Gonzalo (3) Armstrong,Eileen (2) Artigas,Rody (2) Iriarte,Andrés (2) Llambí,Silvia (2) de Bethencourt,Miguel (2) Arruga,M. Victoria (1) García,Cristina B (1) Iriarte,Wanda (1) Martínez,Amparo (1) Tellechea,Beatriz (1) Vega-Pla,José Luis (1) de Soto,Leticia (1) Mais... Palavra-chave Uruguayan Creole cattle (2) 29 (1) 29) Uruguayan Creole cattle (1) 5-azacitidina-C (1) Aphidicolin Break point rob(1 (1) Bisulfite (1) Bovino Criollo (1) Collagen (1) Creole cattle (1) Cytosines (1) DGAT1 gene (1) Descondensación (1) Genetic diversity (1) Methylation (1) Microsatellites (1) Milk protein (1) Rob1 (1) SNPs (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Journal article (1) Ano 2011 (1) 2008 (2) 2006 (2) País Brazil (2) Argentina (1) Chile (1) Colombia (1) Idioma Inglês (4) Espanhol (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Analysis of the population structure of Uruguayan Creole cattle as inferred from milk major gene polymorphisms Genet. Mol. Biol. Rincón,Gonzalo; Armstrong,Eileen; Postiglioni,Alicia. The ancestors of Uruguayan Creole cattle were introduced by the Spanish conquerors in the XVII century, following which the population grew extensively and became semi-feral before the introduction of selected breeds. Today the Uruguayan Creole cattle genetic reserve consists of 575 animals. We used the tetra primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to analyze the kappa-casein, beta-casein, alphaS1-casein and alpha-lactoalbumin gene polymorphisms and restriction fragment length polymorphism PCR (RFLP-PCR) for the beta-lactoglobulin and the acylCoA:diacyl glycerol acyltransferase 1 (DGAT1) genes. The kappa-casein and beta-lactoglobulin genes presented very similar A and B allele frequencies, while the... Tipo: Info:eu-repo/semantics/article Palavras-chave: DGAT1 gene; Milk protein; SNPs; Uruguayan Creole cattle. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300016 Aphidicolin induces break points in heterozygous Robertsonian translocation rob(1;29) from Uruguayan Creole cattle: Brief post JBAG Artigas,Rody; Iriarte,Andrés; Tellechea,Beatriz; Llambí,Silvia; de Bethencourt,Miguel; Postiglioni,Alicia. Bovine presents a complex genome due to high number of repetitive sequences involved in macro and micro-rearrangements that are essential in the bovids evolution. However, it has a morphologically simple karyotype, with acrocentric autosomes (2n=60,XX and 60,XY). The Robertsonian translocation rob(1;29), which produces embryo mortality, has been widely described in different breeds of Bos taurus but the complexity of its mechanism and its consequences in chromatin changes, are still not clear. Clastogenic agents are used as an approach to study this rearrangement in chromatin structure. Particularly, the aphidicolin (APC) inhibits the eukaryotic DNA polymerase alpha during replication. This fact permit to identify regions rich in dCTP due to a competition... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aphidicolin Break point rob(1; 29) Uruguayan Creole cattle. Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332008000100001 Descondensación de la heterocromatina en bovinos criollos portadores de la translocación Robertsoniana (rob1; 29). Acción del inductor 5-azacitidina-C Acta Agron. (Palmira) Artigas,Rody; Iriarte,Wanda; de Soto,Leticia; Iriarte,Andrés; Llambí,Silvia; de Bethencourt,Miguel; Postiglioni,Alicia. La translocación Robertsoniana (rob1; 29) está ampliamente distribuida en razas comerciales y en reservas genéticas de bovinos Criollos americanos. Se ha descrito un enlentecimiento en el desarrollo de embriones portadores de esta aneuploidía, frente a embriones normales. La acción de la 5-aza-C, como agente desmetilante, permitiría descondensar la heterocromatina constitutiva o facultativa. En este trabajo se realiza inducción con el análogo de base 5-aza-C(10mM, 2 hrs) en cultivos linfocitarios de una hembra y un macho portadores de la rob1; 29, frente a bovinos Criollos normales. Se controla la acción desmetilante del inductor al identificar la despiralización del cromosoma X de replicación tardía en hembras y permitir el análisis de la despiralización... Tipo: Info:eu-repo/semantics/article Palavras-chave: 5-azacitidina-C; Descondensación; Rob1; 29; Bovino Criollo. Ano: 2008 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122008000100010 Methylation-specific PCR analysis in Col8A1 promoter in Creole cattle carrier of rob(1;29) Electron. J. Biotechnol. Postiglioni,Alicia; García,Cristina B; Rincón,Gonzalo; Arruga,M. Victoria. Robertsonian translocation (rob(1;29)) is the most frequent structural chromosomal abnormality in cattle. Heterozygous carriers have a normal phenotype but show a 3-5% decrease in fertility. Chromatin decondensation was evaluated similar to the inactive X chromosome when submitted to demethylating agent. Based on this result, and the concept that imprinted genes are essential in embryonic development, we decided to query genes located on BTA1 and BTA29 that could undergo genome imprinting. The collagen typeVIII-α1 (Col8A1) acted on extracellular matrix structural proteins. DNA bisulfite conversion and sequentiation methods were used to compare its differential methylation patterns. It was performed on eight Creole cattle DNA blood samples from normal and... Tipo: Journal article Palavras-chave: Bisulfite; Collagen; Creole cattle; Cytosines; Methylation. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300013 Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus) Genet. Mol. Biol. Armstrong,Eileen; Postiglioni,Alicia; Martínez,Amparo; Rincón,Gonzalo; Vega-Pla,José Luis. The Uruguayan Creole cattle genetic reserve consists of a herd of about 600 animals (bulls, cows and calves) located in an indigenous habitat of 650 hectares. In a previous study, a random sample from this herd showed high heterozygosity and a Hardy-Weinberg equilibrium for markers of major genes related to milk production. To study its genetic diversity we genotyped a sample of bulls (N = 19 out of 23 for the whole herd) using the PCR reaction with a set of 17 microsatellite markers. Between two and seven different alleles were identified per microsatellite in a total of 73 alleles. The expected mean heterozygosity (He) per locus was between 0.465 and 0.801, except for microsatellite HEL13 which gave a He value of 0.288. The expected mean heterozygosity... Tipo: Info:eu-repo/semantics/article Palavras-chave: Uruguayan Creole cattle; Genetic diversity; Microsatellites. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200012 Registros recuperados: 5 Primeira ... 1 ... Última

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